{"id":2000,"date":"2022-03-01T09:31:58","date_gmt":"2022-03-01T04:01:58","guid":{"rendered":"https:\/\/www.kewaunee.in\/blog\/?p=2000"},"modified":"2024-07-17T17:43:21","modified_gmt":"2024-07-17T12:13:21","slug":"pcr-workstations-what-are-they-how-to-use-them","status":"publish","type":"post","link":"https:\/\/www.kewaunee.in\/blog\/pcr-workstations-what-are-they-how-to-use-them\/","title":{"rendered":"PCR Workstations \u2014 What are they & how to use them?"},"content":{"rendered":"

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PCR Workstations \u2014 What are they & how to use them?<\/span><\/h1>[vc_column_text]PCR workstations are a much-needed tool in any biological laboratory these days. Often, they\u2019re confused with biosafety cabinets and other laminar hoods. Still, the biggest differentiator is that they\u2019re meant explicitly for PCR purposes. This is to prevent any cross-contamination in critical experiments such as RNA\/ DNA identification and amplification.<\/span><\/p>\n

At Kewaunee International, we have a range of PCR workstations with varying sizes to fit your laboratory needs. If you\u2019re looking to elevate your experiments, you can view the products <\/span>here<\/span><\/a>.\u00a0<\/span>[\/vc_column_text][vc_row_inner][vc_column_inner][vc_column_text]<\/p>\n\n\n\n\n\n\n\n
The W\u2019s<\/strong><\/td>\nKey Pointers<\/strong><\/td>\n<\/tr>\n
What are they?<\/span><\/td>\n\n
    \n
  • They are a type of workspace which are used explicitly for Polymerase Chain Reaction (PCR) experiments<\/span><\/li>\n
  • Alternatively known as PCR hoods<\/span><\/li>\n
  • Specifically made with a three-sided enclosure to allow only a single-entry point<\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Why do you need to use them?<\/span><\/td>\n\n
    \n
  • Since PCR experiments focus on DNA\/ RNA amplification, it\u2019s easy for cross-contamination to occur<\/span><\/li>\n
  • When this happens, you get improper results or amplification of non-target genes, leading to wasted time & effort<\/span><\/li>\n
  • Air quality within the hood is sustained, providing an aseptic environment<\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
How to use them?<\/span><\/td>\n\n
    \n
  • It\u2019s always important to sterilise the entire hood before using it using ethanol<\/span><\/li>\n
  • Ensure that no open flames must be present within the hood<\/span><\/li>\n
  • Any material that blocks the air grills must be removed<\/span><\/li>\n
  • Regular checks must be performed to ensure that the ventilator and HEPA filters are giving optimal performance (99.99% efficiency at 0.3 microns)<\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
What safety standards need to be followed?<\/span><\/td>\n\n